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Somatic Recombination with MADM (Mosaic Analysis with Double Markers)(双标记嵌合分析下的体细胞重组)
项目编号: 状态:已结束 联系方式:
发布时间:2013/5/6来源:本站 我要咨询 T:
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项目名称:Somatic Recombination with MADM (Mosaic Analysis with Double Markers)( 双标记嵌合分析下的体细胞重组)

编号: 03-250
类别: Genomics/Gene

项目描述:
Stanford scientists in Dr. Liqun Luo's laboratory have developed a patented method for somatic cell recombination and concurrent labeling of "knock in" cells. The technique, mosaic analysis with double markers (MADM), allows for highly sensitive detection of recombination events down to the single-cell level. This enables researchers to study gene function in particular tissues and developmental stages, mimic loss of heterozygosity in cancers, and generate specific mutant mouse models.
Stage of Research
The inventors have used MADM to achieve tumor-suppressor gene inactivation and concurrent labeling of sporadic mutant cells in mice, closely mimicking the loss of heterozygosity that occurs in human cancers.
Ongoing Research
The inventors are continuing to develop the MADM technology on several other mouse chromosomes with improved transgenes.
适用:
  • Research of mutant mouse models:
  • uniquely mark homozygous mutant cells and their wild type siblings with two different colors in the same animal
  • analyze naturally occurring mutants instead of just mutations made through gene targeting
  • identify individual labeled mutant cells due to efficiency of mitotic recombination
  • generate conditional knock out mice to mimic sporadic loss of tumor suppressor genes in human cancers Research of wild type mice:
  • study stem cell behavior
  • label individual cells to analyze normal biological processes
优势:
  • Specific labels - with either GFP or RFP in a regulated manner
  • Efficient
  • Cost effective
  • Permanent label - marker stays on permanently (controlled by a ubiquitous promoter) during the life span of the cell
权利状况:Issued: 7,282,621 (USA)
项目联系方式:0755-86674700, 电子邮箱:ss@sotcbb.com

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